Gender differences in organizational commitment remain inconsistently reported across studies, highlighting the need for further empirical investigation. This study aims to examine differences in organizational commitment between male and female employees. A cross-sectional comparative design was employed using a quantitative approach. The sample consisted of 85 employees (45 females and 40 males). Data were collected using an organizational commitment scale based on Meyer and Allen’s framework and analyzed using the Rasch model to obtain logit measures, followed by a Welch t-test to assess group differences. The results revealed a significant difference in organizational commitment between female and male employees, with females demonstrating higher levels of commitment, t (81) = 3.50, p = 0.001, and a moderate-to-large effect size (Cohen’s d ≈ 0.77). These findings indicate that gender is significantly associated with variations in organizational commitment. This study contributes to the literature by integrating Rasch measurement with inferential statistical analysis to provide a more robust examination of gender differences in organizational commitment. The findings also suggest the importance of developing targeted human resource strategies to enhance employee engagement and organizational attachment.